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KMID : 0903519750180030177
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1975 Volume.18 No. 3 p.177 ~ p.182
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Studies on the Hydrolysis of Inulin in Jerusalem Artichokes by Fungal Inulase
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Abstract
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The analysis of Jerusalem artichoke showed that it contains 12.09% of Inulin. The results obtained from the examination of the conditions for fructose production by cultivating Pencillum sp 1 in the Jerusalem articoke medium were as follows:
1. The optimum amount of water added to Jerusalem artichoke was 2.5 L of distilled water per ㎏ of fresh Jerusalem artichoke. It this case, the concentration of Inulin was 4% (w/v).
2. The optimum temperature was 30℃, the initial optimum pH was 5.0 and the optimum cultural period was 72 hours.
3. Shaking culture with 50 ㎖ of the medium and 120 oscills/min in 500 ㎖ shaking flask was most effective as the culture method.
4. 0.1% of NH₄H₂PO₄ as a nitrogen source, 0.001 of FeSO₄7H₂O and 0.001 of MgSO₄7H₂ as metal salts were most effective.
5. Fructose production continued to increase for 72 hours under the optimum conditions for cultivation and the highest production rate to the Inulin was 95.25%.
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